FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13935064
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT194503913.957876332681357RNA PCR Primer, Index 12 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC191815913.764981631946577RNA PCR Primer, Index 12 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT10166417.295560321789695RNA PCR Primer, Index 12 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG9330096.695405202301188RNA PCR Primer, Index 12 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT8779516.30030116833335RNA PCR Primer, Index 12 (100% over 38bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT7445025.342652175835001RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC5411753.883548722847631RNA PCR Primer, Index 12 (100% over 51bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC3507952.517354782152418RNA PCR Primer, Index 12 (100% over 42bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC3064862.19938710005207RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT2732531.9609023682991338RNA PCR Primer, Index 12 (100% over 36bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA1550881.11293353227513RNA PCR Primer, Index 12 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1102160.7909256821497197No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC909220.6524691956922479RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT722050.518153343249805RNA PCR Primer, Index 12 (100% over 38bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG713320.5118885711612089RNA PCR Primer, Index 12 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC712270.5111350762364636RNA PCR Primer, Index 2 (100% over 34bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT603680.4332093487335258RNA PCR Primer, Index 12 (100% over 41bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT547270.3927287309193557RNA PCR Primer, Index 12 (100% over 36bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC455380.32678716079093717RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACCTTGT442910.31783851154181997RNA PCR Primer, Index 12 (97% over 38bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT339090.24333580383986753RNA PCR Primer, Index 12 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA289010.20739768400059017No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT284760.20434782359090709RNA PCR Primer, Index 12 (100% over 46bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT273990.19661911850566313RNA PCR Primer, Index 12 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACCTTG272560.19559293017958151RNA PCR Primer, Index 12 (97% over 37bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG238170.17091417735864003RNA PCR Primer, Index 12 (100% over 45bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT233490.16755574283691843RNA PCR Primer, Index 1 (97% over 35bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT228950.16429777430516285RNA PCR Primer, Index 12 (100% over 43bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC216230.15516972150253489RNA PCR Primer, Index 12 (100% over 44bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC211600.15184716769151546RNA PCR Primer, Index 12 (100% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCG203950.14635741895408588RNA PCR Primer, Index 12 (98% over 51bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACCTTGTAATCTCGT201140.14434092301262486RNA PCR Primer, Index 12 (97% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT197160.14148481844073338RNA PCR Primer, Index 12 (100% over 46bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC194810.13979842503773215RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT190810.13692796818155986RNA PCR Primer, Index 12 (100% over 43bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT187780.13475359711300933RNA PCR Primer, Index 12 (100% over 43bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC187440.13450960828023467RNA PCR Primer, Index 12 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG175240.1257547148689091RNA PCR Primer, Index 12 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT162880.11688500318333667RNA PCR Primer, Index 12 (100% over 46bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA160630.11527037120173972No Hit
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC154140.11061305495260014RNA PCR Primer, Index 12 (100% over 42bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTC152580.10949357677869295RNA PCR Primer, Index 12 (97% over 39bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACCTTGTA144480.10368090164494399RNA PCR Primer, Index 12 (97% over 39bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT143840.10322162854795644RNA PCR Primer, Index 12 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT143160.10273365088240714RNA PCR Primer, Index 12 (100% over 46bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTCG142230.10206626966334709RNA PCR Primer, Index 12 (97% over 39bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC140580.100882206210176RNA PCR Primer, Index 12 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)