FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13022028
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT184345814.156458579262768RNA PCR Primer, Index 13 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC154938111.898154419572743RNA PCR Primer, Index 13 (100% over 44bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC8299916.373746086247088RNA PCR Primer, Index 13 (100% over 37bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT8065196.193497664111919RNA PCR Primer, Index 13 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC7834326.016205770714055RNA PCR Primer, Index 13 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3624172.7831072088003497RNA PCR Primer, Index 1 (100% over 33bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC3556402.731064623728347RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC3019662.318886121270819RNA PCR Primer, Index 13 (100% over 42bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1738961.3353987566299197RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT1572381.207477053497351RNA PCR Primer, Index 8 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1412861.0849769329324128No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA1320691.0141968670317711RNA PCR Primer, Index 13 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC1281390.9840172360249878RNA PCR Primer, Index 13 (100% over 51bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT1017780.7815833294168927RNA PCR Primer, Index 8 (97% over 36bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA757860.5819830828193582RNA PCR Primer, Index 13 (100% over 38bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC539170.41404457124497046RNA PCR Primer, Index 1 (100% over 33bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT505610.3882728558101703RNA PCR Primer, Index 13 (100% over 41bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG495810.3807471462970284RNA PCR Primer, Index 13 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG490070.37633923072504527RNA PCR Primer, Index 13 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT407770.3131386294054966RNA PCR Primer, Index 13 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT407540.3129620056108004RNA PCR Primer, Index 13 (100% over 46bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT393180.3019345373854211RNA PCR Primer, Index 13 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG376450.28908707614512885RNA PCR Primer, Index 13 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACAGTC370680.284656122686881RNA PCR Primer, Index 13 (97% over 37bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT352480.2706798050196175RNA PCR Primer, Index 13 (100% over 43bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC350700.2693128904345775RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTC323650.24854039631922153RNA PCR Primer, Index 13 (97% over 39bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC319680.2454917160368569RNA PCR Primer, Index 1 (100% over 33bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT317520.2438329882257971RNA PCR Primer, Index 13 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA271070.20816266099258884No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC263950.20269500265242862RNA PCR Primer, Index 13 (100% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTCG262900.20188867663316346RNA PCR Primer, Index 13 (97% over 39bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT252770.19410955037110963RNA PCR Primer, Index 13 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACAGTC251360.1930267697166678RNA PCR Primer, Index 13 (97% over 37bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC226030.17357511441382248RNA PCR Primer, Index 13 (100% over 42bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG224110.1721006896928804RNA PCR Primer, Index 13 (100% over 45bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT220960.1696817116350848RNA PCR Primer, Index 13 (100% over 46bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG213150.163684181910836RNA PCR Primer, Index 13 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT205260.15762521782321462RNA PCR Primer, Index 13 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC195930.1504604351948867RNA PCR Primer, Index 13 (100% over 42bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT194720.1495312404488763RNA PCR Primer, Index 13 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT191050.14671293902915888RNA PCR Primer, Index 13 (100% over 43bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC189990.14589893371447213RNA PCR Primer, Index 13 (100% over 44bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT178980.13744402945531986RNA PCR Primer, Index 13 (100% over 43bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC177940.13664538273147625RNA PCR Primer, Index 13 (100% over 44bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT176610.13562403644040696RNA PCR Primer, Index 13 (100% over 43bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC175820.13501737210210268RNA PCR Primer, Index 13 (100% over 42bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT156290.12001970814376993RNA PCR Primer, Index 13 (100% over 43bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC153120.11758537149513118RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG152440.11706317940646419RNA PCR Primer, Index 13 (100% over 45bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC151630.11644115647731675RNA PCR Primer, Index 1 (100% over 31bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC150030.11521246920986501RNA PCR Primer, Index 13 (100% over 44bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC145460.11170303120220598RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT144990.11134210431739204RNA PCR Primer, Index 13 (100% over 46bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACAGTCAAATCTCGT144870.11124995277233315RNA PCR Primer, Index 13 (97% over 46bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC138870.10664237551938915RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA137470.10556727416036887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)