FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12650113
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT180324814.254797565839924RNA PCR Primer, Index 15 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC171231513.535966042358675RNA PCR Primer, Index 15 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT8827216.977969287705177RNA PCR Primer, Index 15 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT7223795.710454918465945RNA PCR Primer, Index 15 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTC6264554.952169201966813RNA PCR Primer, Index 15 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC3701322.925918527368095RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT3287702.5989491161067098RNA PCR Primer, Index 1 (100% over 35bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC3059512.4185633756789366RNA PCR Primer, Index 15 (100% over 42bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1719211.359047148432587RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1492081.179499345183715RNA PCR Primer, Index 15 (100% over 36bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA1266311.0010266311455085RNA PCR Primer, Index 15 (100% over 39bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG1218810.963477559449469RNA PCR Primer, Index 15 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC1154450.912600543568267RNA PCR Primer, Index 15 (100% over 51bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC668470.5284300622452938No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG593500.46916576950735533RNA PCR Primer, Index 15 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC540190.427023853462811RNA PCR Primer, Index 1 (100% over 33bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAAT531070.41981443169717136RNA PCR Primer, Index 15 (100% over 41bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG497940.39362494232264955RNA PCR Primer, Index 15 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTC486250.3843839181515612RNA PCR Primer, Index 15 (100% over 38bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT464540.3672220161195398RNA PCR Primer, Index 15 (100% over 46bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT443410.35051860801559637RNA PCR Primer, Index 1 (100% over 35bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT425190.3361155746197682RNA PCR Primer, Index 15 (100% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT396090.31311182753861566RNA PCR Primer, Index 15 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG387290.30615536794019155RNA PCR Primer, Index 15 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT375860.2971198755299656RNA PCR Primer, Index 15 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT312490.24702546135358636RNA PCR Primer, Index 15 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC305310.24134962272669025RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACATGTC292800.2314603830021123RNA PCR Primer, Index 15 (97% over 38bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT283030.2237371318343164RNA PCR Primer, Index 15 (100% over 43bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC282690.22346835953165003RNA PCR Primer, Index 15 (100% over 44bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACATGT256140.2024804047204954RNA PCR Primer, Index 15 (97% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG236680.18709714292670745RNA PCR Primer, Index 15 (100% over 45bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT229440.1813738738934585RNA PCR Primer, Index 15 (100% over 46bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT214070.1692237847993927RNA PCR Primer, Index 15 (100% over 46bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC212420.16791944862468816RNA PCR Primer, Index 15 (100% over 42bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG211660.16731866347755156RNA PCR Primer, Index 15 (100% over 45bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC209720.16578508033880804RNA PCR Primer, Index 15 (100% over 44bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT204530.1616823501892829RNA PCR Primer, Index 15 (100% over 43bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC200500.15849660789591366RNA PCR Primer, Index 15 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACATGTCAATCTCGT177740.1405046737527167RNA PCR Primer, Index 15 (97% over 46bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT174900.1382596345186798RNA PCR Primer, Index 15 (100% over 46bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC174870.13823591931550336RNA PCR Primer, Index 15 (100% over 42bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT171130.13527942398617307RNA PCR Primer, Index 15 (100% over 43bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC164800.130275516115943RNA PCR Primer, Index 1 (100% over 31bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG164240.12983283232331602RNA PCR Primer, Index 15 (100% over 45bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC163500.12924785731163035RNA PCR Primer, Index 15 (100% over 44bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC163020.1288684140608072RNA PCR Primer, Index 15 (100% over 42bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT158110.12498702580759555RNA PCR Primer, Index 15 (100% over 43bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT152580.12061552335540403RNA PCR Primer, Index 15 (100% over 43bp)
TCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT145470.11499502020258634RNA PCR Primer, Index 15 (100% over 46bp)
TCCCGTGGTGGAATTCTCGGGTGCCAGGAACTCCAGTCACATGTCAATCTC136620.1079990352655348RNA PCR Primer, Index 15 (100% over 26bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT129750.10256825373812865RNA PCR Primer, Index 15 (100% over 43bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT129170.10210975981005072RNA PCR Primer, Index 15 (100% over 43bp)
GAATTCCACCACGTTCCCGTGATGGAATTCTCGGGTGCCAAGGAACTCC128520.10159593040789437RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)