FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004879359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11835696
Sequences flagged as poor quality0
Sequence length35-51
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT178330815.067200103821524RNA PCR Primer, Index 18 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC155163513.10979092399805RNA PCR Primer, Index 18 (100% over 44bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG9105587.693320274532228RNA PCR Primer, Index 18 (100% over 38bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC8167736.900929189124154RNA PCR Primer, Index 18 (100% over 37bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT7133066.02673471843143RNA PCR Primer, Index 18 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT5443554.599264800312546RNA PCR Primer, Index 1 (97% over 35bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC3071062.594743900147486RNA PCR Primer, Index 18 (100% over 42bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2914492.4624576366273687RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC2239731.8923517467836282RNA PCR Primer, Index 1 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC1826671.5433566391025926RNA PCR Primer, Index 18 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC1387071.1719378395660043RNA PCR Primer, Index 18 (100% over 39bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC890640.752503274839097RNA PCR Primer, Index 1 (97% over 36bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC787470.6653347635829782RNA PCR Primer, Index 18 (100% over 39bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG568510.4803350812660278RNA PCR Primer, Index 6 (100% over 34bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCAT557770.47126083670956065RNA PCR Primer, Index 18 (100% over 41bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCG500250.42266208932706617RNA PCR Primer, Index 18 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGTCCG439220.371097736880028RNA PCR Primer, Index 18 (97% over 38bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT389880.3294102856308577RNA PCR Primer, Index 1 (97% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC366110.30932697156128375RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC363720.3073076564318651RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCG339250.28663291115283795RNA PCR Primer, Index 18 (100% over 45bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGTCC338140.28569507023499086RNA PCR Primer, Index 18 (97% over 37bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT327600.2767898060240817RNA PCR Primer, Index 18 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC315500.2665664951178199No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT303110.2560981627104988RNA PCR Primer, Index 18 (100% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT275880.23309148866277066RNA PCR Primer, Index 18 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCG255390.21577945226034867RNA PCR Primer, Index 18 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT248600.21004256952865297RNA PCR Primer, Index 18 (100% over 43bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT240320.20304678322254985RNA PCR Primer, Index 18 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT221270.1869514053081458RNA PCR Primer, Index 18 (100% over 46bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT220920.1866556897034192RNA PCR Primer, Index 18 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT205630.1737371422855065RNA PCR Primer, Index 18 (100% over 43bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC200110.16907328474810437RNA PCR Primer, Index 18 (100% over 42bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC186720.1577600506129931RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC181040.15296100879914457RNA PCR Primer, Index 18 (100% over 44bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCCG180750.1527159872980854RNA PCR Primer, Index 18 (98% over 51bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT168560.1424166352363224RNA PCR Primer, Index 18 (100% over 43bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC167390.1414281002148078RNA PCR Primer, Index 18 (100% over 44bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT167110.14119152773102656RNA PCR Primer, Index 18 (100% over 43bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT157110.13274251045312419RNA PCR Primer, Index 18 (100% over 46bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC152490.1288390644707333RNA PCR Primer, Index 18 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCG150870.1274703236717131RNA PCR Primer, Index 18 (100% over 45bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC149280.12612692992452662RNA PCR Primer, Index 18 (100% over 42bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCG143610.12133633712795597RNA PCR Primer, Index 18 (100% over 45bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC143460.12120960186878743RNA PCR Primer, Index 18 (100% over 50bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC139970.11826089483879951RNA PCR Primer, Index 18 (100% over 44bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGT135630.11459402134018988RNA PCR Primer, Index 18 (100% over 46bp)
GAATTCCACCACGTTCCCGTGATGGAATTCTCGGGTGCCAAGGAACTCC129960.10980342854361924RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGTCCGCATCTCGT129360.1092964875069451RNA PCR Primer, Index 18 (97% over 46bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCG120210.10156563669766441RNA PCR Primer, Index 18 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)