FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17773340
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT13995727.874558186587327RNA PCR Primer, Index 6 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC11928206.711287805218377RNA PCR Primer, Index 6 (100% over 36bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC11221656.31375419589115RNA PCR Primer, Index 6 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC10086835.675258561418394No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC7712454.339336331831833RNA PCR Primer, Index 5 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC4475752.518237990158293RNA PCR Primer, Index 6 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3287661.849770498960803No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC3154911.7750799793398429RNA PCR Primer, Index 6 (100% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2607031.4668205300748198RNA PCR Primer, Index 6 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT2545071.4319593278472138RNA PCR Primer, Index 6 (100% over 39bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC2361521.32868667341085RNA PCR Primer, Index 6 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG2022541.1379628139674367RNA PCR Primer, Index 6 (100% over 45bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT1939091.0910104684881965RNA PCR Primer, Index 6 (100% over 46bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1610790.9062956090414069RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT1443250.8120308281954882RNA PCR Primer, Index 6 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1348010.7584449518210984RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT1129260.6353673535756363RNA PCR Primer, Index 6 (100% over 46bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1066370.5999828957303467RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG1016700.5720365446224513RNA PCR Primer, Index 6 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC932690.5247691204917028RNA PCR Primer, Index 6 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA859260.48345443231266605No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC820780.46180402783044716RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA765850.43089818796016954No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA726680.4088595615680564RNA PCR Primer, Index 6 (100% over 37bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC713370.4013708171902411RNA PCR Primer, Index 1 (100% over 31bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT683630.3846378902333495RNA PCR Primer, Index 6 (100% over 46bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC682370.3839289632674556No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC673190.37876392394451464RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT632610.3559319745191393RNA PCR Primer, Index 6 (100% over 39bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC600550.3378937217202844RNA PCR Primer, Index 6 (100% over 44bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG581880.3273892245351746RNA PCR Primer, Index 6 (100% over 45bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC566930.318977749820799RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG561050.315669423979961RNA PCR Primer, Index 6 (100% over 45bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC544550.3063858565694461RNA PCR Primer, Index 6 (100% over 44bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC540000.3038258425259405No Hit
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG515800.29020994365718544RNA PCR Primer, Index 6 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT514050.2892253228712217RNA PCR Primer, Index 6 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT513370.28884272736581873RNA PCR Primer, Index 6 (100% over 43bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG461240.259512280753083RNA PCR Primer, Index 6 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG458650.25805504198985674RNA PCR Primer, Index 6 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT437690.24626209817625722RNA PCR Primer, Index 6 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT426490.2399605251460896RNA PCR Primer, Index 6 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT403680.2271266965016142RNA PCR Primer, Index 6 (100% over 43bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT398190.22403780043593383RNA PCR Primer, Index 6 (100% over 41bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC353220.19873585943891245RNA PCR Primer, Index 1 (100% over 31bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC345570.19443166000312828RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC339010.19074073865688723RNA PCR Primer, Index 6 (100% over 44bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG312330.17572949147430927No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC306650.1725336937232957No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT297710.167503688108144RNA PCR Primer, Index 6 (100% over 46bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT293830.1653206431655502RNA PCR Primer, Index 1 (100% over 30bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG285560.16066760665131033RNA PCR Primer, Index 6 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG280330.15772499710240168Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT268640.15114773025216421RNA PCR Primer, Index 6 (100% over 43bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA266600.14999994373595507No Hit
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC256880.14453107857048816RNA PCR Primer, Index 6 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC253190.14245493531322756RNA PCR Primer, Index 6 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC244300.13745306172053198RNA PCR Primer, Index 6 (100% over 44bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC242960.13669912351870836RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC239780.13490992689050005RNA PCR Primer, Index 1 (100% over 27bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC236110.1328450364422219RNA PCR Primer, Index 1 (100% over 31bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC224120.12609897745724777RNA PCR Primer, Index 6 (100% over 44bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC216260.12167662352714796No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC214820.12086642128041213No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCC203210.11433416566610441RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT200000.11252808982442242RNA PCR Primer, Index 6 (100% over 43bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT199610.11230866004926479RNA PCR Primer, Index 1 (100% over 30bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG199420.1122017583639316RNA PCR Primer, Index 6 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA199400.11219050555494915No Hit
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC197750.11126214881389768RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC194040.10917475274765463RNA PCR Primer, Index 6 (100% over 42bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT185570.10440918814359035RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC181920.10235555050429462No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA181240.1019729549988916No Hit
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC181040.10186042690906719RNA PCR Primer, Index 6 (100% over 44bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA180620.10162411792043588No Hit
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG178000.10014999994373595RNA PCR Primer, Index 6 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC578500.059.24829545
ATATGCC1050.053.5492445
ATAAATC352.9184504E-547.24933245
AATGTCT7900.046.4718145
TATGTCG4450.045.33812345
TCCCACA36750.044.9019431
ACATACG10150.044.802661
GTTTCCG1159500.044.787591
TTCACAG71900.044.742661
CACTAGA5450.044.611391
ATTACAC254.0997966E-544.6074453
CTCCATA502.3646862E-1144.6074453
GCACATA302.3032553E-644.6039356
TACCATT207.349306E-444.6038068
CACATAA302.303299E-644.6038067
GTAGGCC2600.044.5234145
TATTGCA39000.044.4472161
TTCCGTA1167150.044.376223
GCATTGG413800.044.3661771
TTTGTCA109250.044.3589676
Produced by FastQC (version 0.11.5)