FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004879362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19174627
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT18127659.453977905280766RNA PCR Primer, Index 8 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC16235478.467163402969977RNA PCR Primer, Index 8 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC13408876.993027817438118RNA PCR Primer, Index 8 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT12658206.601536499249764RNA PCR Primer, Index 8 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC9489724.949102790891317RNA PCR Primer, Index 5 (100% over 35bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT3445931.7971301345262154RNA PCR Primer, Index 8 (100% over 36bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT2982271.5553209978999851RNA PCR Primer, Index 8 (100% over 46bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC2858671.4908608130943044RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2851901.4873301055608539RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA2484651.2958009561281165RNA PCR Primer, Index 8 (100% over 39bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC2423331.2638211945400555RNA PCR Primer, Index 8 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG2348361.2247226504067068RNA PCR Primer, Index 8 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2273811.18584314573629No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT1811030.9444929489371553RNA PCR Primer, Index 8 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT1367890.7133854546427422RNA PCR Primer, Index 8 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1341710.6997319947866522RNA PCR Primer, Index 1 (100% over 33bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG1271640.6631889110541759RNA PCR Primer, Index 8 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC1184220.6175974114124879RNA PCR Primer, Index 8 (100% over 44bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1078630.5625298473863404RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG893140.4657926331500477RNA PCR Primer, Index 8 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG833160.4345117117532456RNA PCR Primer, Index 8 (100% over 38bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT782990.4083469263834963RNA PCR Primer, Index 8 (100% over 46bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC720380.37569440073071564RNA PCR Primer, Index 8 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA714510.3726330634749766No Hit
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC711390.37100591317891085RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC698360.3642104746027133RNA PCR Primer, Index 8 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG679570.3544110662491635RNA PCR Primer, Index 8 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG673240.351109828629261RNA PCR Primer, Index 8 (100% over 38bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT658600.343474738778491RNA PCR Primer, Index 8 (100% over 43bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG648820.33837424842736186RNA PCR Primer, Index 8 (100% over 45bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT621830.32429835532133167RNA PCR Primer, Index 8 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT617890.3222435565500179RNA PCR Primer, Index 8 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG529450.2761201039269239RNA PCR Primer, Index 8 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT499940.2607299740433021RNA PCR Primer, Index 8 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG497330.2593688002379394RNA PCR Primer, Index 8 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT491350.25625009550381345RNA PCR Primer, Index 8 (100% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT427910.2231647061504769RNA PCR Primer, Index 8 (100% over 41bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC412060.21489857403745063RNA PCR Primer, Index 8 (100% over 44bp)
TTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTGGAATTCTCGGGTG410880.21428317745111808No Hit
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC348840.18192791964088792RNA PCR Primer, Index 8 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC341000.17783918299949197RNA PCR Primer, Index 8 (100% over 44bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT335040.1747309087159818RNA PCR Primer, Index 1 (100% over 30bp)
GGTCTTATGGTCTAGTGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCA332710.1735157612192404No Hit
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG329870.17203463723179596RNA PCR Primer, Index 8 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT322010.16793547013978422RNA PCR Primer, Index 8 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT321560.16770078500092858RNA PCR Primer, Index 8 (100% over 43bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC318050.16587024091785463RNA PCR Primer, Index 8 (100% over 44bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC314900.16422744494586516RNA PCR Primer, Index 1 (100% over 27bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC296040.15439153001516015RNA PCR Primer, Index 8 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC279050.14553086221703296RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT241350.1258694628062387RNA PCR Primer, Index 8 (100% over 43bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG239950.12513933126313226RNA PCR Primer, Index 8 (100% over 45bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG231110.12052907209094602RNA PCR Primer, Index 8 (100% over 45bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG228020.11891756747080398RNA PCR Primer, Index 8 (100% over 45bp)
TTTGACCTCGGATCAGGTAGGGATACCCGCTTGGAATTCTCGGGTGCCA219810.11463586749301563No Hit
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC216650.1129878562957183RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC214940.11209605276806688RNA PCR Primer, Index 8 (100% over 42bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC214200.11171012609528207RNA PCR Primer, Index 8 (100% over 44bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC213240.11120946446572337RNA PCR Primer, Index 8 (100% over 44bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC208820.10890433487963025RNA PCR Primer, Index 8 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA200170.10439316498829417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)