FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004879367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8099551
Sequences flagged as poor quality0
Sequence length40-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC499915461.72137196247051RNA PCR Primer, Index 11 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG2318152.8620722309174917RNA PCR Primer, Index 2 (97% over 35bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC1268921.5666547441950796RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACGTCTCGTATGCC930731.1491130804658185RNA PCR Primer, Index 11 (98% over 51bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC884051.0914802561277779RNA PCR Primer, Index 11 (98% over 51bp)
CACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCACGG685750.8466518699616806RNA PCR Primer, Index 11 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT682460.842589916404008RNA PCR Primer, Index 11 (100% over 37bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC625870.7727218459393613RNA PCR Primer, Index 11 (98% over 51bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG460080.5680314871774992RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCAAAGGAACTCCAGTCACGGCTACATCTCGTATGCC445840.5504502656999135RNA PCR Primer, Index 11 (98% over 51bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG355380.438765062409015RNA PCR Primer, Index 11 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG301650.372428051875962RNA PCR Primer, Index 6 (100% over 34bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT293290.3621064920759188RNA PCR Primer, Index 11 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT292620.36127928572830764RNA PCR Primer, Index 11 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC275160.3397225352368298RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT273450.33761130709591186RNA PCR Primer, Index 11 (100% over 43bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT251860.3109555085213983RNA PCR Primer, Index 11 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACGGCTACATCTCGTATGCC243710.30089322235269583RNA PCR Primer, Index 11 (98% over 51bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC230450.284521944488034RNA PCR Primer, Index 6 (97% over 36bp)
TGGAATTCTCGGGTGCCAACGAACTCCAGTCACGGCTACATCTCGTATGCC219940.2715459165575968RNA PCR Primer, Index 11 (98% over 51bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC216390.2671629575515976RNA PCR Primer, Index 11 (100% over 39bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC209030.2580760340912725RNA PCR Primer, Index 11 (98% over 51bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT208570.25750810137500213RNA PCR Primer, Index 11 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAATCTCGTATGCCG204730.2527670978304847RNA PCR Primer, Index 11 (97% over 38bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC197100.24334682255843565RNA PCR Primer, Index 11 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGCTACATCTCGTATGCCG186630.23042018008158724TruSeq Adapter, Index 11 (96% over 33bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGCTACATCTCGTATGCCG185280.2287534210229678RNA PCR Primer, Index 11 (97% over 36bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT168110.2075547150700082RNA PCR Primer, Index 11 (100% over 41bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT166900.20606080509894933RNA PCR Primer, Index 11 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG164690.20333225878817235RNA PCR Primer, Index 11 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACGGCTACATCTCGTATGCCG143190.17678757748423338Illumina PCR Primer Index 11 (96% over 32bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC139430.17214534484689337RNA PCR Primer, Index 11 (100% over 44bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG139340.17203422757631873RNA PCR Primer, Index 11 (100% over 45bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG134700.1663055149600268RNA PCR Primer, Index 11 (100% over 51bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT131860.1627991477552274RNA PCR Primer, Index 11 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCATCACGGCTACATCTCGTATGCCG130380.16097188597244463RNA PCR Primer, Index 1 (96% over 28bp)
GAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGT128850.15908289237267598RNA PCR Primer, Index 11 (100% over 51bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT127590.15752725058463116RNA PCR Primer, Index 11 (100% over 46bp)
TGGAATTCTCGGGTGACAAGGAACTCCAGTCACGGCTACATCTCGTATGCC126860.15662596605663695RNA PCR Primer, Index 11 (98% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC126350.15599630152338073RNA PCR Primer, Index 11 (100% over 39bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT123640.15265043704274472RNA PCR Primer, Index 11 (100% over 46bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG116060.1432918935876816RNA PCR Primer, Index 11 (97% over 42bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG111560.1377360300589502RNA PCR Primer, Index 11 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC111300.13741502461062347RNA PCR Primer, Index 11 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG110330.13621742736109693RNA PCR Primer, Index 11 (100% over 45bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC104270.1287355311424053RNA PCR Primer, Index 11 (98% over 51bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG100020.12348832669860342RNA PCR Primer, Index 11 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTACATCTCGTATGCC98080.12109313219955031RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACTACATCTCGTATGCC95980.11850039588614232RNA PCR Primer, Index 11 (98% over 51bp)
TTTAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC86660.10699358519996972RNA PCR Primer, Index 11 (100% over 48bp)
TGGAATTCTCGGGTGCCAAGGAATCCAGTCACGGCTACATCTCGTATGCCG83730.10337610072459573TruSeq Adapter, Index 11 (96% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC81900.10111671622291162RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)