FastQCFastQC Report
Wed 8 May 2024
EGAF00004879368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26573839
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1551548958.38632874986561RNA PCR Primer, Index 12 (100% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC6468162.434032960010031RNA PCR Primer, Index 2 (100% over 34bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC3775561.4207807912134938RNA PCR Primer, Index 12 (98% over 50bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC3661701.377934140415316RNA PCR Primer, Index 12 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAGTCTCGTATGC3455711.3004180540116916RNA PCR Primer, Index 12 (98% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT2643690.994846849188783RNA PCR Primer, Index 12 (100% over 36bp)
CACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCACC2361700.8887312066577959RNA PCR Primer, Index 2 (97% over 34bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1784310.6714536051791388RNA PCR Primer, Index 12 (98% over 50bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC1774960.6679351071555751RNA PCR Primer, Index 12 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC1541200.5799688934669921RNA PCR Primer, Index 12 (100% over 44bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1403530.5281623027820708RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC1339350.504010730252411RNA PCR Primer, Index 12 (100% over 42bp)
TGGAATTCTCGGGTGCAAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1279660.48154878939395995RNA PCR Primer, Index 12 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTCGTATGCC1211310.4558280043767858RNA PCR Primer, Index 12 (97% over 39bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC1160680.4367754316566756RNA PCR Primer, Index 12 (98% over 50bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1141620.4296029640278923RNA PCR Primer, Index 12 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT1115010.4195893562838248RNA PCR Primer, Index 12 (100% over 43bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT978500.3682192851397948RNA PCR Primer, Index 12 (100% over 38bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT922210.3470367981080942RNA PCR Primer, Index 12 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA921130.3466303833631264RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG883080.33231178980199283RNA PCR Primer, Index 12 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT776850.2923363839150226RNA PCR Primer, Index 12 (100% over 35bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC725110.2728661071514733RNA PCR Primer, Index 12 (100% over 44bp)
TGGAATTCTCGGGTGCCAACGAACTCCAGTCACCTTGTAATCTCGTATGC718020.2701980696127496RNA PCR Primer, Index 12 (98% over 50bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG703310.2646625502623087RNA PCR Primer, Index 12 (100% over 45bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC651860.24530140338398226RNA PCR Primer, Index 12 (98% over 50bp)
TGGAATTCTCGGGTGACAAGGAACTCCAGTCACCTTGTAATCTCGTATGC643710.24223447729927167RNA PCR Primer, Index 12 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACCTTGTAATCTCGTATGC640580.24105662715876316RNA PCR Primer, Index 12 (98% over 50bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC617150.2322396850526565RNA PCR Primer, Index 12 (100% over 44bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT611020.22993290506501526RNA PCR Primer, Index 12 (100% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA558700.2102443685310203RNA PCR Primer, Index 12 (100% over 40bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA557120.2096497988115304RNA PCR Primer, Index 12 (100% over 39bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG544420.20487066245866847RNA PCR Primer, Index 12 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG512660.1929190584770232RNA PCR Primer, Index 12 (100% over 45bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACCTTGTAATCTCGTATGCC505230.19012307555562447RNA PCR Primer, Index 12 (97% over 35bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC490650.18463647649855935RNA PCR Primer, Index 1 (100% over 27bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC464160.17466802594837727RNA PCR Primer, Index 12 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC461480.1736595152849387RNA PCR Primer, Index 12 (100% over 44bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT461390.1736256473895247RNA PCR Primer, Index 12 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC434760.16360451344647645RNA PCR Primer, Index 12 (100% over 44bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT430120.16185843528291113RNA PCR Primer, Index 12 (100% over 46bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC417320.1570416679351448RNA PCR Primer, Index 12 (98% over 50bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC379640.1428623090551576RNA PCR Primer, Index 12 (97% over 41bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC369790.13915565605707175RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACCTTGTAATCTCGTATGCC362050.1362430170514693TruSeq Adapter, Index 12 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACCTTGTAATCTCGTATGCC336210.12651916796816598Illumina PCR Primer Index 12 (96% over 31bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC330380.12432528096523804RNA PCR Primer, Index 12 (100% over 42bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT326990.12304959023797804RNA PCR Primer, Index 12 (100% over 41bp)
TTTAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC323190.1216196124316099RNA PCR Primer, Index 12 (100% over 47bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC312290.11751783398702763RNA PCR Primer, Index 12 (100% over 50bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT312230.11749525539008496RNA PCR Primer, Index 12 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC306680.1154067351728894RNA PCR Primer, Index 12 (100% over 42bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC297030.11177534416461242RNA PCR Primer, Index 12 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG281560.10595382925289794RNA PCR Primer, Index 12 (100% over 45bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT279290.1050996056685675RNA PCR Primer, Index 12 (100% over 43bp)
GGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG268270.1009526700300999RNA PCR Primer, Index 12 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)