FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004879368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26573839
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC1551417458.38138027403568RNA PCR Primer, Index 12 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT6463922.432437405826083RNA PCR Primer, Index 1 (97% over 35bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC3774971.4205587683435579RNA PCR Primer, Index 12 (98% over 51bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC3660801.377595461461176RNA PCR Primer, Index 12 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAGTCTCGTATGCC3452901.2993606230548773RNA PCR Primer, Index 12 (98% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG2642250.9943049628621593RNA PCR Primer, Index 12 (100% over 37bp)
CACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCACCT2359540.8879183771678605RNA PCR Primer, Index 12 (97% over 35bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC1783790.6712579240056358RNA PCR Primer, Index 12 (98% over 51bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT1774920.6679200547576134RNA PCR Primer, Index 12 (100% over 43bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1541190.5799651303675016RNA PCR Primer, Index 12 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1400960.5271951862130271RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT1339270.5039806254564875RNA PCR Primer, Index 12 (100% over 43bp)
TGGAATTCTCGGGTGCAAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC1278870.481251504534215RNA PCR Primer, Index 12 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTATCTCGTATGCCG1210830.4556473756012446RNA PCR Primer, Index 12 (97% over 39bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCG1160640.43676037925871375RNA PCR Primer, Index 12 (98% over 51bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT1141610.4295992009284018RNA PCR Primer, Index 12 (100% over 46bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC1114930.41955925148790135RNA PCR Primer, Index 12 (100% over 44bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT976910.36762095232081443RNA PCR Primer, Index 12 (100% over 38bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC922150.3470142195111515RNA PCR Primer, Index 12 (100% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC919700.34609226013599315RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT881990.33190161195753465RNA PCR Primer, Index 12 (100% over 38bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT776540.29221972783081884RNA PCR Primer, Index 12 (100% over 36bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG725110.2728661071514733RNA PCR Primer, Index 12 (100% over 45bp)
TGGAATTCTCGGGTGCCAACGAACTCCAGTCACCTTGTAATCTCGTATGCC717440.26997980984230396RNA PCR Primer, Index 12 (98% over 51bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT703300.2646587871628183RNA PCR Primer, Index 12 (100% over 46bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC651050.24499659232525645RNA PCR Primer, Index 12 (98% over 51bp)
TGGAATTCTCGGGTGACAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC642330.2417151695695906RNA PCR Primer, Index 12 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACCTTGTAATCTCGTATGCC639720.24073300060258512RNA PCR Primer, Index 12 (98% over 51bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG617130.23223215885367557RNA PCR Primer, Index 12 (100% over 45bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT610660.22979743348335932RNA PCR Primer, Index 12 (100% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT554190.20854721066083073RNA PCR Primer, Index 12 (100% over 41bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA552850.20804295532911146RNA PCR Primer, Index 12 (100% over 39bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT544410.204866899359178RNA PCR Primer, Index 12 (100% over 46bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT512660.1929190584770232RNA PCR Primer, Index 12 (100% over 46bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACCTTGTAATCTCGTATGCCG505220.19011931245613403RNA PCR Primer, Index 12 (97% over 36bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC489540.18421877245512025RNA PCR Primer, Index 1 (100% over 27bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG464150.1746642628488868RNA PCR Primer, Index 12 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG461480.1736595152849387RNA PCR Primer, Index 12 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC461350.17361059499156295RNA PCR Primer, Index 12 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG434760.16360451344647645RNA PCR Primer, Index 12 (100% over 45bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT429860.16176059469615964RNA PCR Primer, Index 12 (100% over 46bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC416980.15691372255246974RNA PCR Primer, Index 12 (98% over 51bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCG379610.14285101975668626RNA PCR Primer, Index 12 (97% over 42bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC369790.13915565605707175RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACCTTGTAATCTCGTATGCCG362000.1362242015540171TruSeq Adapter, Index 12 (96% over 33bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACCTTGTAATCTCGTATGCCG336200.12651540486867555Illumina PCR Primer Index 12 (96% over 32bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT330370.12432151786574759RNA PCR Primer, Index 12 (100% over 43bp)
TTTAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC322720.12144274675555909RNA PCR Primer, Index 12 (100% over 48bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC321930.12114546189581415RNA PCR Primer, Index 12 (100% over 42bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCG312260.1175065446885563RNA PCR Primer, Index 12 (100% over 51bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC312220.11749149229059452RNA PCR Primer, Index 12 (100% over 44bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT306670.11540297207339896RNA PCR Primer, Index 12 (100% over 43bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG297030.11177534416461242RNA PCR Primer, Index 12 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT281560.10595382925289794RNA PCR Primer, Index 12 (100% over 46bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC279290.1050996056685675RNA PCR Primer, Index 12 (100% over 44bp)
GGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT268270.1009526700300999RNA PCR Primer, Index 12 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)