FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004879369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339453
Sequences flagged as poor quality0
Sequence length45-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC139177259.49134263436795RNA PCR Primer, Index 22 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG28350812.118559338443644RNA PCR Primer, Index 2 (100% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC454721.9437022244088682RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT317041.3551885846819747RNA PCR Primer, Index 12 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT234261.0013451862465286RNA PCR Primer, Index 12 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACCGTACGATCTCGTATGCC189850.8115144865060337RNA PCR Primer, Index 22 (98% over 51bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC173150.7401302783171964RNA PCR Primer, Index 2 (100% over 34bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT97170.41535350357540846RNA PCR Primer, Index 22 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGACCTCGTATGCC94430.40364136402825784RNA PCR Primer, Index 22 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAGCTCGTATGCC93420.3993241155090528RNA PCR Primer, Index 22 (98% over 51bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT88680.37906296899317915RNA PCR Primer, Index 22 (100% over 43bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG70970.30336151228513675RNA PCR Primer, Index 22 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATATCGTATGCC69970.2990870088007752RNA PCR Primer, Index 22 (98% over 51bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC69730.29806112796452844RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG66830.28566506785988005RNA PCR Primer, Index 22 (100% over 39bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC64610.27617567012459754RNA PCR Primer, Index 1 (100% over 31bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC62030.2651474511349448RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAACTCGTATGCC59350.2536917817968559RNA PCR Primer, Index 22 (98% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA57580.24612591062953604No Hit
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT55080.23543965191863228RNA PCR Primer, Index 1 (100% over 30bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAT54580.2333024001764515RNA PCR Primer, Index 22 (100% over 41bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACCGTACGATCTCGTATGCCG49540.21175890261526947RNA PCR Primer, Index 22 (97% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC48380.2068004785734101RNA PCR Primer, Index 22 (100% over 38bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC45180.19312206742345325No Hit
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC44740.1912412858903342RNA PCR Primer, Index 1 (100% over 27bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC32010.13682685653441212RNA PCR Primer, Index 22 (100% over 44bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCG31820.1360147008723834RNA PCR Primer, Index 22 (97% over 42bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATC31630.13520254521035474RNA PCR Primer, Index 22 (100% over 42bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA29710.1269954985203806No Hit
TGGAATTCTTGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC27000.1154115940777609RNA PCR Primer, Index 22 (98% over 51bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG26270.112291206534177RNA PCR Primer, Index 22 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT26100.11156454094183556RNA PCR Primer, Index 22 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCTACGATCTCGTATGCC25930.11083787534949409RNA PCR Primer, Index 22 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACCGTACGATCTCGTATGCCG25000.10686258710903787RNA PCR Primer, Index 22 (100% over 32bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG24430.10442612012295181RNA PCR Primer, Index 2 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)