FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004879372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374437
Sequences flagged as poor quality0
Sequence length45-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC47295334.41067142400852RNA PCR Primer, Index 25 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC20169114.674444881795237RNA PCR Primer, Index 5 (100% over 35bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT420333.058197647473111RNA PCR Primer, Index 25 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA343712.500733027414134No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC299142.1764547956727007RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG277042.0156616854755804RNA PCR Primer, Index 5 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC250761.8244561227615381No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT173761.2642267342919318RNA PCR Primer, Index 8 (100% over 36bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC142351.0356967980343952RNA PCR Primer, Index 25 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA113470.8255743988265741No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC110260.8022193814631009RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC88490.6438272543594213No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC86590.6300034123062752RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACACTGATATCTCGTATGCC74850.5445866198305197RNA PCR Primer, Index 25 (98% over 51bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA71240.518321319929542No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT63810.4642628217953969RNA PCR Primer, Index 25 (100% over 39bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC60550.4405440191147357RNA PCR Primer, Index 1 (100% over 27bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT55970.4072212840603098RNA PCR Primer, Index 25 (100% over 46bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC53960.3925971143093499No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG53760.3911419730405977RNA PCR Primer, Index 25 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT43800.318675937856737RNA PCR Primer, Index 25 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT42600.3099450902442237RNA PCR Primer, Index 25 (100% over 46bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC41120.2991770448554572No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT39640.28840899946669074RNA PCR Primer, Index 25 (100% over 39bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA37150.2702924906707255No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT36940.2687645923385357RNA PCR Primer, Index 25 (100% over 41bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA36230.2635988408344653No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC33620.24460924727724878RNA PCR Primer, Index 25 (100% over 42bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG32880.23922522458286558RNA PCR Primer, Index 5 (97% over 37bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC32730.2381338686313014No Hit
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC28960.210704455715322RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC25740.18727668128841118RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC24350.17716344947058324RNA PCR Primer, Index 25 (100% over 44bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC24240.17636312177276953No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC23860.1735983533621403RNA PCR Primer, Index 1 (100% over 31bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT22850.16624988995494155RNA PCR Primer, Index 1 (100% over 30bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT22110.16086586726055832RNA PCR Primer, Index 25 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG21830.1588286694843052RNA PCR Primer, Index 25 (100% over 45bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC21230.15446324567804853RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC21180.1540994603608605RNA PCR Primer, Index 1 (100% over 27bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA21170.15402670329742288No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC20320.14784235290522593No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATACCTCGTATGCC19750.14369520028928207RNA PCR Primer, Index 25 (98% over 51bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT19620.14274935846459313RNA PCR Primer, Index 25 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG19400.14114870306896568RNA PCR Primer, Index 25 (100% over 45bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC18790.1367105221992714RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAGCTCGTATGCC18530.13481883854989352RNA PCR Primer, Index 25 (98% over 51bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC17900.13023514355332402RNA PCR Primer, Index 25 (100% over 44bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG17630.1282707028405085RNA PCR Primer, Index 25 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC17170.1249238779223784No Hit
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG16260.11830298514955578RNA PCR Primer, Index 25 (100% over 45bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTGC16210.11793919983236772No Hit
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT14820.10782596801453977RNA PCR Primer, Index 25 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT14620.10637082674578756RNA PCR Primer, Index 25 (100% over 46bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT14290.10396984365234638RNA PCR Primer, Index 25 (100% over 43bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC14240.10360605833515833No Hit
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT14100.10258745944703175RNA PCR Primer, Index 25 (100% over 43bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACACTGATATCTCGTATGCCG13830.10062301873421627RNA PCR Primer, Index 25 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)