FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004879373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308585
Sequences flagged as poor quality0
Sequence length45-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC82251062.8549158060042RNA PCR Primer, Index 26 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT679385.191714714749137RNA PCR Primer, Index 1 (100% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA346692.6493502523718364No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA258561.9758747043562321No Hit
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACATGAGCATCTCGTATGCC119810.9155691070889548RNA PCR Primer, Index 26 (98% over 51bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC83150.6354191741461197RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG66500.5081825024740464RNA PCR Primer, Index 15 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC60810.4647004206834099No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG58520.44720060217716084RNA PCR Primer, Index 15 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC55550.4245043310140343No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC54410.415792630971622No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCACCTCGTATGCC50480.3857601913517273RNA PCR Primer, Index 26 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAGCTCGTATGCC45680.3490793490678863RNA PCR Primer, Index 26 (98% over 51bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC43390.3315795305616372RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA42520.324931127897691No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA37640.28763893824245274No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC32720.2500410749015157RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA31330.2394189143234868No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCT30270.23131856165247194RNA PCR Primer, Index 26 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAACTCGTATGCC29470.22520508793849844RNA PCR Primer, Index 26 (98% over 51bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGT27250.20824019838222202RNA PCR Primer, Index 26 (100% over 46bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA26400.20174463256112518No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC25670.19616608779712438RNA PCR Primer, Index 1 (100% over 27bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC23970.1831749561549307RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTTGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC23470.17935403508369727RNA PCR Primer, Index 26 (98% over 51bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCG23410.17889552455514926RNA PCR Primer, Index 26 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAAATGAGCATCTCGTATGCC21510.16437602448446223RNA PCR Primer, Index 26 (98% over 51bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCCG20510.15673418234199538RNA PCR Primer, Index 26 (97% over 42bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGT20210.1544416296992553RNA PCR Primer, Index 26 (100% over 46bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC20090.15352460864215928RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCT19730.1507735454708712RNA PCR Primer, Index 26 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCA18750.1432845401712537No Hit
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT18300.1398457112071436RNA PCR Primer, Index 1 (100% over 30bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC16780.12823011115059396RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC16340.12486770060790855RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACATGAGCATCTCGTATGCCG15890.12142887164379845RNA PCR Primer, Index 26 (97% over 36bp)
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTTGGAATTCTCGGGTGCCA15780.1205882690081271No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCCG15760.12043543216527777RNA PCR Primer, Index 26 (98% over 51bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCC15460.11814287952253771RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACATGAGCATCTCGTATGCCG14310.1093547610587008RNA PCR Primer, Index 26 (96% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC14000.10698578999453609No Hit
TGGAATTCTCGGGTGCAAGGAACTCCAGTCACATGAGCATCTCGTATGCCG13340.10194217418050795RNA PCR Primer, Index 26 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)