FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004879376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881545
Sequences flagged as poor quality0
Sequence length45-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCC69948137.17588471176613RNA PCR Primer, Index 19 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT38746620.592970138901805RNA PCR Primer, Index 1 (97% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC643203.418467270248652RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG473612.5171335259055723RNA PCR Primer, Index 15 (97% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG302341.6068709491402013RNA PCR Primer, Index 15 (97% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG230221.2235689287261267RNA PCR Primer, Index 6 (100% over 34bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT201371.0702374909980894RNA PCR Primer, Index 19 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACGTGAAAATCTCGTATGCC185280.9847226614298356RNA PCR Primer, Index 19 (98% over 51bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC143290.7615549986845916RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA95460.507349013709478RNA PCR Primer, Index 19 (100% over 39bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA74270.3947288000021259RNA PCR Primer, Index 19 (100% over 38bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG70880.37671169172143104RNA PCR Primer, Index 19 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC70520.37479837048808295No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT68290.36294640840373205RNA PCR Primer, Index 19 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA67390.3581631053203617No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAATCTCGTATGCCG65600.34864964696565853RNA PCR Primer, Index 19 (97% over 39bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA65180.34641743886008575RNA PCR Primer, Index 19 (100% over 37bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT61950.32925069557199005RNA PCR Primer, Index 19 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC61740.32813459151920366RNA PCR Primer, Index 19 (100% over 44bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT58580.31133988291537007RNA PCR Primer, Index 19 (100% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAT51270.27248883231599563RNA PCR Primer, Index 19 (100% over 41bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC48580.2581920708779221RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC45150.23996237134907747RNA PCR Primer, Index 19 (100% over 42bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC39800.21152829190904282RNA PCR Primer, Index 1 (100% over 31bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT38740.20589462383307336RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAGCTCGTATGCC36490.19393636612464757RNA PCR Primer, Index 19 (98% over 51bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA34860.18527327276254354No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAACCTCGTATGCC34040.18091515217547283RNA PCR Primer, Index 19 (98% over 51bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC33370.1773542487689638RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC33020.17549407534765313RNA PCR Primer, Index 19 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT31560.1677344947901857RNA PCR Primer, Index 19 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAACTCGTATGCC30540.16231341796236604RNA PCR Primer, Index 19 (98% over 51bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG30150.16024065329290557RNA PCR Primer, Index 19 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG26390.14025707596682513RNA PCR Primer, Index 19 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG25960.13797172004921487RNA PCR Primer, Index 19 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC25880.1375465375529153RNA PCR Primer, Index 19 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC22900.1217084895657558RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT22750.12091127238519409RNA PCR Primer, Index 19 (100% over 46bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGTGAAAATCTCGTATGCCG22710.1206986811370443RNA PCR Primer, Index 19 (97% over 36bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG21160.11246077027123987RNA PCR Primer, Index 19 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT20780.11044115341381684RNA PCR Primer, Index 19 (100% over 46bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT19730.10486063314988481RNA PCR Primer, Index 19 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCAC19730.10486063314988481RNA PCR Primer, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)