FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004879378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599248
Sequences flagged as poor quality0
Sequence length45-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC86628854.168459175812636RNA PCR Primer, Index 21 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1526659.546049143097255RNA PCR Primer, Index 1 (97% over 35bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT507043.17049013036127RNA PCR Primer, Index 21 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC300921.8816343681530316RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT193181.2079427330845498RNA PCR Primer, Index 21 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACGTTTCGATCTCGTATGCC161301.0085990415495283RNA PCR Primer, Index 21 (98% over 51bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC158230.9894025191840164RNA PCR Primer, Index 21 (100% over 44bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT125440.7843686532670355RNA PCR Primer, Index 9 (97% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG87430.5466944463898032RNA PCR Primer, Index 6 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCATCACGTTTCGATCTCGTATGCCG78760.4924814662891559RNA PCR Primer, Index 1 (96% over 28bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC60620.379053154982842RNA PCR Primer, Index 1 (100% over 27bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT59630.37286274549038045RNA PCR Primer, Index 21 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATATCGTATGCC55560.34741328424359447RNA PCR Primer, Index 21 (98% over 51bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGTTTCGATCTCGTATGCCG51770.3237146458835653RNA PCR Primer, Index 21 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAGCTCGTATGCC50980.3187748241673587RNA PCR Primer, Index 21 (98% over 51bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG50530.3159610016707853RNA PCR Primer, Index 21 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA50380.31502306083859416No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGACCTCGTATGCC48220.30151671285504184RNA PCR Primer, Index 21 (98% over 51bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT47870.2993281842465959RNA PCR Primer, Index 21 (100% over 43bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG47740.29851530219203026RNA PCR Primer, Index 21 (100% over 39bp)
GAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT47660.2980150670815283RNA PCR Primer, Index 1 (100% over 30bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC46460.2905115404239993RNA PCR Primer, Index 1 (100% over 27bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC41820.261497904014887RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT38870.24305173431512808RNA PCR Primer, Index 21 (100% over 46bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC37980.2374866187107941RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG37970.23742408932198134RNA PCR Primer, Index 21 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC37250.2329219733274639No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAT33060.20672215941492503RNA PCR Primer, Index 21 (100% over 41bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC30340.1897141656578592RNA PCR Primer, Index 21 (100% over 38bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC28840.1803347573359479RNA PCR Primer, Index 21 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC27860.1742068772322992RNA PCR Primer, Index 21 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA25600.1600752353606195No Hit
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGTTTCGATCTCGTATGCCG25480.15932488269486658RNA PCR Primer, Index 21 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAACTCGTATGCC25180.15744900103048434RNA PCR Primer, Index 21 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACGTTTCGATCTCGTATGCCG22860.1429421828259282RNA PCR Primer, Index 21 (96% over 32bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT22080.13806489049853432RNA PCR Primer, Index 21 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG21770.13612647944533932RNA PCR Primer, Index 21 (100% over 45bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCCG21640.13531359739077367RNA PCR Primer, Index 21 (97% over 42bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT21070.13174942222844735RNA PCR Primer, Index 21 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAAGTTTCGATCTCGTATGCC20950.13099906956269447RNA PCR Primer, Index 21 (98% over 51bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG17990.11249037047412283RNA PCR Primer, Index 21 (100% over 45bp)
TGGAATTCTCAGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC17870.11174001780836994RNA PCR Primer, Index 21 (98% over 51bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT16610.10386131481796444RNA PCR Primer, Index 21 (100% over 37bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT16510.10323602092983702RNA PCR Primer, Index 1 (97% over 35bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC16400.10254819765289686RNA PCR Primer, Index 21 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG16360.10229808009764589RNA PCR Primer, Index 21 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)