FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22949974
Sequences flagged as poor quality0
Sequence length35-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC1312769057.20132841980562RNA PCR Primer, Index 5 (100% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGGTCTCGTATGCC15343416.685589273434471RNA PCR Primer, Index 5 (98% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC5283122.3020156798434717RNA PCR Primer, Index 5 (100% over 35bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT2206010.9612254898415137RNA PCR Primer, Index 5 (100% over 43bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC2165520.9435827683290621RNA PCR Primer, Index 5 (98% over 51bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC2078710.9057570174153574RNA PCR Primer, Index 5 (98% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC1712770.7463058563813624RNA PCR Primer, Index 5 (100% over 35bp)
CACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCACAC1625470.7082665976005027RNA PCR Primer, Index 5 (97% over 35bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC1623400.7073646357943586RNA PCR Primer, Index 5 (98% over 51bp)
TGGAATTCTCGGGTGCAAAGGAACTCCAGTCACACAGTGATCTCGTATGCC1121810.4888066539857518RNA PCR Primer, Index 5 (98% over 51bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1078040.4697347369543861RNA PCR Primer, Index 1 (100% over 33bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1067770.4652597863509562RNA PCR Primer, Index 5 (100% over 45bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCCG1058740.4613251413705305RNA PCR Primer, Index 5 (98% over 51bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1050150.4575822177402031RNA PCR Primer, Index 5 (100% over 44bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC862240.37570412933801145RNA PCR Primer, Index 5 (100% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT852550.3714819023324384RNA PCR Primer, Index 5 (100% over 38bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT828770.36112023482030964RNA PCR Primer, Index 5 (100% over 43bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG780640.34014853350160656RNA PCR Primer, Index 5 (100% over 39bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT763190.33254503904884597RNA PCR Primer, Index 5 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC750370.32695897607552843RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAACGAACTCCAGTCACACAGTGATCTCGTATGCC629940.274483971092952RNA PCR Primer, Index 5 (98% over 51bp)
TGGAATTCTCGGGTGACAAGGAACTCCAGTCACACAGTGATCTCGTATGCC530370.23109830102639767RNA PCR Primer, Index 5 (98% over 51bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC498410.21717235932380577RNA PCR Primer, Index 5 (100% over 44bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC490900.21390002446190137RNA PCR Primer, Index 5 (98% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG469870.20473661538788673RNA PCR Primer, Index 5 (100% over 39bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACACAGTGATCTCGTATGCCG457400.19930305803396556RNA PCR Primer, Index 5 (97% over 36bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG455410.19843595465511202RNA PCR Primer, Index 5 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT435270.18966034558470524RNA PCR Primer, Index 5 (100% over 46bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT435050.1895644849096561RNA PCR Primer, Index 5 (100% over 46bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC400650.17457536117470113RNA PCR Primer, Index 5 (100% over 42bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG386980.16861892741142104RNA PCR Primer, Index 5 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACACAGTGATCTCGTATGCC384290.16744681279377485RNA PCR Primer, Index 5 (98% over 51bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG357450.15575181043778089RNA PCR Primer, Index 5 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAGCTCGTATGCC348510.1518563811880571RNA PCR Primer, Index 5 (98% over 51bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCCG348010.15163851601749093RNA PCR Primer, Index 5 (97% over 42bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACACAGTGATCTCGTATGCCG332670.1449544125845197TruSeq Adapter, Index 5 (96% over 33bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGGTCTCGTATGCC331040.14424417212847387RNA PCR Primer, Index 5 (97% over 39bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC327220.14257968222534806RNA PCR Primer, Index 5 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACACAGTGATCTCGTATGCCG319760.13932913388050025Illumina PCR Primer Index 5 (96% over 32bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT318500.13878011365067341RNA PCR Primer, Index 5 (100% over 46bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC316590.1379478686991105RNA PCR Primer, Index 5 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT314780.13715919678166086RNA PCR Primer, Index 5 (100% over 46bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCCG313880.13676703947464167RNA PCR Primer, Index 5 (100% over 51bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG307760.1341003697869113RNA PCR Primer, Index 5 (100% over 45bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGGTCTCGTATGCC306400.13350777652297124RNA PCR Primer, Index 5 (97% over 39bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACACAGTGGTCTCGTATGCC287260.12516789779369683RNA PCR Primer, Index 5 (97% over 39bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG271560.11832693143791796RNA PCR Primer, Index 5 (100% over 45bp)
ACCACGTTCCCGTGGTGGACTTCTCGGGTGCCAAGGAACTCCAGTCACAC266460.11610470669814266RNA PCR Primer, Index 5 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAACTCGTATGCC237320.10340752455754416RNA PCR Primer, Index 5 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGGT252.9388306E-547.19394345
GTATGCC17418800.045.29982445
CATTATT800.045.1291051
ATTTTCC206.937483E-445.1291051
AATGACA253.8278202E-545.1291051
TGAGGTA4350.045.1291051
TATAATT453.7471182E-1045.1291051
CCCATAA502.1827873E-1145.1291051
GATCTCA207.0869044E-444.9343139
AGGTGAT850.044.93264435
ACACTAA253.927717E-544.9326434
GTGTTTT207.088806E-444.9318637
GTGATCA850.044.93185837
TCACTAA253.9281214E-544.93185830
ACACTTT502.1827873E-1144.93185832
CACAAGC502.1827873E-1144.93185831
TATCACT700.044.93107620
TTATCAC13950.044.93107619
GGAACGA253.9285253E-544.93107620
AAACACT253.9285253E-544.93107620