EGAF00004879380 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004879380
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21154013
Sequences flagged as poor quality	0
Sequence length	40-51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	11742636	55.51020508496426	RNA PCR Primer, Index 9 (100% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGGTCTCGTATGCC	1641103	7.757880266028011	RNA PCR Primer, Index 9 (98% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	587716	2.7782719051935914	RNA PCR Primer, Index 6 (100% over 34bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	272429	1.2878360243042302	RNA PCR Primer, Index 9 (98% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	211150	0.9981557636369043	RNA PCR Primer, Index 9 (100% over 37bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	178295	0.8428424431808754	RNA PCR Primer, Index 9 (98% over 51bp)
CACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCACG	169956	0.803422026827723	RNA PCR Primer, Index 6 (97% over 34bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT	158791	0.7506424431146941	RNA PCR Primer, Index 9 (100% over 43bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	135193	0.6390891411478286	RNA PCR Primer, Index 9 (98% over 51bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	130707	0.6178827629537715	RNA PCR Primer, Index 6 (100% over 34bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	112714	0.5328256156408716	RNA PCR Primer, Index 9 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	107709	0.5091658022522724	RNA PCR Primer, Index 9 (100% over 37bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG	100798	0.4764958781106923	RNA PCR Primer, Index 9 (98% over 51bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT	89714	0.4240992004684879	RNA PCR Primer, Index 9 (100% over 36bp)
TGGAATTCTCGGGTGCAAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	89392	0.42257703065607455	RNA PCR Primer, Index 9 (98% over 51bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	89050	0.42096031613481566	RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC	69485	0.3284719547066554	RNA PCR Primer, Index 9 (100% over 44bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	66867	0.3160960523187728	RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG	64838	0.30650449160639165	RNA PCR Primer, Index 9 (100% over 45bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	58448	0.2762974571302381	RNA PCR Primer, Index 9 (100% over 37bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT	56804	0.2685258820631338	RNA PCR Primer, Index 9 (100% over 43bp)
TGGAATTCTCGGGTGCCAACGAACTCCAGTCACGATCAGATCTCGTATGCC	54365	0.2569961548194189	RNA PCR Primer, Index 9 (98% over 51bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT	53231	0.25163546982787616	RNA PCR Primer, Index 9 (100% over 46bp)
TGGAATTCTCGGGTGACAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	47249	0.22335714741217186	RNA PCR Primer, Index 9 (98% over 51bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGGTCTCGTATGCC	46017	0.21753319334728594	RNA PCR Primer, Index 9 (97% over 39bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT	44851	0.2120212368215903	RNA PCR Primer, Index 9 (100% over 46bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	43761	0.20686855018950778	RNA PCR Primer, Index 9 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACGATCAGATCTCGTATGCC	36393	0.1720382794508068	RNA PCR Primer, Index 9 (98% over 51bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	34360	0.16242780979665655	RNA PCR Primer, Index 9 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAGCTCGTATGCC	33316	0.15749257599491878	RNA PCR Primer, Index 9 (98% over 51bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC	32860	0.1553369566332402	RNA PCR Primer, Index 9 (100% over 44bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGGTCTCGTATGCC	32580	0.1540133307094025	RNA PCR Primer, Index 9 (97% over 39bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG	31633	0.14953663874556566	RNA PCR Primer, Index 9 (100% over 45bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGATCAGATCTCGTATGCCG	31394	0.14840682947486133	RNA PCR Primer, Index 9 (97% over 36bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	29934	0.14150506572913613	RNA PCR Primer, Index 9 (98% over 51bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG	27613	0.13053315226760992	RNA PCR Primer, Index 9 (100% over 51bp)
ACCACGTTCCCGTGGTGGACTTCTCGGGTGCCAAGGAACTCCAGTCACGAT	27596	0.1304527892650912	RNA PCR Primer, Index 9 (97% over 36bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACGATCAGGTCTCGTATGCC	27366	0.12936552511336738	RNA PCR Primer, Index 9 (97% over 39bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT	27267	0.1288975288045819	RNA PCR Primer, Index 9 (100% over 46bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG	27231	0.12872734832865992	RNA PCR Primer, Index 9 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAACTCGTATGCC	25382	0.11998668999588873	RNA PCR Primer, Index 9 (98% over 51bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	25200	0.11912633314539421	RNA PCR Primer, Index 9 (100% over 37bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG	24824	0.11734889261909787	RNA PCR Primer, Index 9 (97% over 42bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC	24637	0.11646489959139196	RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG	24279	0.11477254930305658	RNA PCR Primer, Index 9 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT	22690	0.10726097218527757	RNA PCR Primer, Index 9 (100% over 46bp)
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC	22599	0.10683079376003031	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACGATCAGGTCTCGTATGCC	21984	0.10392354396302962	RNA PCR Primer, Index 9 (97% over 39bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	1587250	0.0	48.228146	45
GTAGGCC	390	0.0	46.075226	45
GTATACC	60	1.8189894E-12	45.75526	45
CCCCTTA	20	6.9278665E-4	45.1418	41
TTTCGAG	35	1.2097735E-7	45.016533	1
CATTATT	90	0.0	45.016533	1
CCTCCAC	35	1.2097735E-7	45.016533	1
ACATACG	1435	0.0	45.016533	1
AGTAAAT	35	1.2097735E-7	45.016533	1
GTACTCG	20	7.1688317E-4	44.829563	39
TCATGTC	35	1.250337E-7	44.82808	36
TACCCAC	60	0.0	44.827866	26
ACCAAAG	25	3.9822233E-5	44.827866	34
ATTAGGT	75	0.0	44.827763	35
TTTAAAA	410	0.0	44.827763	24
TCTACCC	20	7.170251E-4	44.827763	24
ATAAGGT	35	1.2504097E-7	44.827763	35
ATCATGT	30	2.2252734E-6	44.827763	35
CTCTACC	25	3.9822786E-5	44.82776	23
CAGGTTT	25	3.9825565E-5	44.827232	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content