FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004879381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25095343
Sequences flagged as poor quality0
Sequence length40-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC1606015963.99657099725634RNA PCR Primer, Index 10 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT9153653.6475492684041018RNA PCR Primer, Index 3 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC3687571.4694240281951914RNA PCR Primer, Index 10 (100% over 37bp)
CACCACGTTCCCGTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCACT3105471.2374686410940867RNA PCR Primer, Index 3 (97% over 34bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC2954791.1774256283327151RNA PCR Primer, Index 10 (98% over 51bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC2712621.0809256522216095RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTGTCTCGTATGCC2620201.044098102185732RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTACCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC2040500.8130990678230618RNA PCR Primer, Index 10 (98% over 51bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1586640.6322447953789673RNA PCR Primer, Index 3 (100% over 34bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT1544940.61562816654867RNA PCR Primer, Index 10 (100% over 43bp)
TGGAATTCTCGGGTGCAAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC1372520.5469221918983136RNA PCR Primer, Index 10 (98% over 51bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG1334320.5317002441448998RNA PCR Primer, Index 10 (98% over 51bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT1276510.5086640975578616RNA PCR Primer, Index 10 (100% over 38bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT1255930.5004633728257869RNA PCR Primer, Index 10 (100% over 39bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1126120.4487366440857174RNA PCR Primer, Index 1 (100% over 33bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1067840.42551321175406925RNA PCR Primer, Index 3 (100% over 34bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT993030.39570289993645436RNA PCR Primer, Index 10 (100% over 41bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT875750.3489691294516277RNA PCR Primer, Index 10 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACTAGCTTATCTCGTATGCC750480.29905150130843RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAACGAACTCCAGTCACTAGCTTATCTCGTATGCC750080.29889210918535764RNA PCR Primer, Index 10 (98% over 51bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC712820.2840447329211639RNA PCR Primer, Index 10 (100% over 44bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC688450.27433376782297814RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGACAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC638510.2544336612573895RNA PCR Primer, Index 10 (98% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT586940.23388403179028078RNA PCR Primer, Index 10 (100% over 39bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG466370.1858392610931837RNA PCR Primer, Index 10 (100% over 45bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG457780.182416315250204RNA PCR Primer, Index 10 (97% over 36bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC399280.15910521725086602RNA PCR Primer, Index 10 (98% over 51bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT380400.15158190904184893RNA PCR Primer, Index 10 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT360390.1436083180851523RNA PCR Primer, Index 10 (100% over 43bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC357980.14264798054364108RNA PCR Primer, Index 10 (100% over 42bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCC350280.13957968217449748RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACTAGCTTATCTCGTATGCCG339080.13511670272847037TruSeq Adapter, Index 10 (96% over 33bp)
TGGAATTCTCGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG338470.13487362974078498RNA PCR Primer, Index 10 (97% over 42bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACTAGCTTATCTCGTATGCCG336300.13400892747311724Illumina PCR Primer Index 10 (96% over 32bp)
ACCACGTTCCCGTGGTGGACTTCTCGGGTGCCAAGGAACTCCAGTCACT327780.1306138752516752RNA PCR Primer, Index 3 (97% over 34bp)
GAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCGT322770.12861748891019342RNA PCR Primer, Index 10 (100% over 51bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC313120.12477215394107186RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG312910.12468847307645886RNA PCR Primer, Index 10 (100% over 51bp)
CACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT301530.12015376717504918RNA PCR Primer, Index 10 (100% over 41bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACTAGCTTATCTCGTATGCC270540.10780486244001528RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAGCTCGTATGCC252030.10042899194484013RNA PCR Primer, Index 10 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)