FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20431206
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN243170.11901891645554354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC53800.034.5409621
CTCGCTC52400.032.741843
TCGCTCT59000.032.0577244
TCTCGCT59850.030.810822
TCTGTCG78350.030.8026358
CTGTCGC79800.029.8863229
CGCTCTG59600.029.341115
CTTGCTC65900.026.9720133
CTATAAT104250.025.666582
GCTAGAG43500.024.6919212
CGTATCA202200.024.41734594-95
GTCTCAC87800.024.3047851
CCTATAA113450.024.046251
GTCTTGC85650.023.9160731
GTCACCC108150.022.367553
CTGTCAC122400.022.3267941
AAGACCA257950.021.7749395
GGCGCAA51800.021.7449571
GTATCAT203500.021.11053594-95
TCAAGAC274450.020.4509683
Produced by FastQC (version 0.11.5)