FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20410911
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN248870.1219298834824178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC51800.038.636161
CTCGCTC49650.037.434723
TCTCGCT57450.034.834382
TCGCTCT58100.034.0350884
CGCTCTG58250.031.3344825
TCTGTCG77350.030.460638
CTGTCGC76450.030.2605259
CTTGCTC64700.028.506543
CTATAAT99450.026.4323862
GTCTTGC81650.026.0833551
GCTAGAG41400.025.834392
CCTATAA109500.025.310231
GTCTCAC84400.024.1633151
CGTATCA207450.023.8582694-95
GGCGCAA51950.023.6089021
CTGTCAC121750.023.0369341
GTCACCC106650.022.7759843
AAGACCA250550.021.7219245
GCGCAAT53650.021.441822
GTATCAT213250.021.3606394-95
Produced by FastQC (version 0.11.5)