FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16811550
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG12350.022.21563343
TATGCCG12800.022.12618446
CTCGTAT15500.018.27145842
CGTATGC15750.017.60723944
CCCGTAT15900.012.2833211
CGTATCG16350.012.267293
ACGTCTG24500.012.15891115
GATCGGA24700.011.6210071
GTATGCC24950.011.58776145
ACACGTC26150.011.50450313
GTCCGAT9150.010.9957991
TTCGCGC4104.1263775E-810.79056557
CGTCTGA27750.010.52238516
GTATCGA13850.010.2223064
CCCGTCT39150.010.2040141
CCGTATC22950.09.2535812
GCCGTCT32250.09.14859449
GTCGATT8800.09.0792521
ATGCCGT42250.09.00849447
TCGGAAG31800.08.997093
Produced by FastQC (version 0.11.5)