FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19733351
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25350.038.17278343
TATGCCG26450.036.36282746
CGTATGC30300.031.74238644
CTCGTAT32700.029.5019242
GATCGGA35250.027.5391671
ACACGTC41500.023.59489813
GTATGCC41750.023.39007845
CGTCTGA43850.022.26331316
ACGTCTG44700.022.0376315
AATCTCG46950.021.1761439
CACGTCT46700.021.09383214
ATCTCGT48500.020.49937840
ATGCCGT51200.020.28503847
TCGGAAG49500.019.7826333
ATCGGAA50950.019.2196352
GCCGTCT51850.018.72158249
CGGAAGA57000.017.7488614
CACACGT57250.017.05216412
TGCCGTC64000.015.85921148
TCTCGTA62550.015.75314341
Produced by FastQC (version 0.11.5)