FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20823569
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17450.024.85406746
TCGTATG18550.023.69742843
CCCGTAT21150.021.268541
GTATCGA17550.021.1758424
CGTATGC23700.019.17063344
CGTATCG23600.018.2468593
CTCGTAT24700.017.67727542
CCGTATC29950.015.4615192
GTCCGAT15650.015.317051
TATCGAA26300.014.9156485
ACGATCA31950.013.84791532
GTATGCC33350.013.80043645
GTCACGA32050.013.712213529
GATCGGA32900.013.6726321
ATCGAAG46900.013.269546
ACGTTCG14000.013.0620066
ACACGTC34900.012.76107613
AGTCACG34450.012.32956228
ACGTCTG36750.012.27913615
CCCGTCT50750.012.1291881
Produced by FastQC (version 0.11.5)