FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27520050
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23800.031.85771646
TCGTATG26750.028.34386843
CGTATGC29150.025.8079244
CTCGTAT36950.020.43947842
GATCGGA39100.019.8285271
TATCTCG40700.018.77365539
CGTCTGA44900.017.3419116
ACGTTCG18800.016.787436
ACACGTC48400.016.2094613
GTATGCC50000.015.5180545
ATGCCGT52300.015.2323347
ACGTCTG51550.015.10453915
TAGCTTA52350.014.65029934
ACTAGCT56450.013.69024932
GTCCGAT21900.013.1067051
CACTAGC60550.013.00670231
CACGTCT66100.012.49363314
CGTTCGA24600.012.4689437
GCGATCG25200.012.2921441
GCTTATC61800.012.17233536
Produced by FastQC (version 0.11.5)