FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25766819
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16350.023.63867646
TCGTATG19250.019.61712343
CCCGTAT29000.019.5910931
CGTATCG29150.019.325863
GTATCGA26750.018.4135154
TATCGAA28050.017.6652415
CGTATGC22700.016.89573144
ACGGCTA24200.016.45474232
CCGTATC38500.016.0881442
CGGCTAC25550.015.70075933
CTCGTAT26400.014.52748742
ACGTTCG15700.013.9019346
ATCGAAG56900.013.01081856
CCCGTCT62150.012.4742821
GTCCGAT16750.011.6596231
GCGATCG17250.011.45855941
AGTCACG36150.011.42314428
CGTTCGA19750.010.9013317
TCGACCG17550.010.58843831
TTCGCGC7800.010.5873487
Produced by FastQC (version 0.11.5)