FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16492207
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24350.029.56359143
TATGCCG25050.028.97354146
CGTATGC27600.026.296444
CTCGTAT28450.025.3024242
GTATGCC35000.021.41075745
ACACGTC40050.018.92616713
GATCGGA40350.018.3331451
GTATCGA18550.017.9673354
CCCGTAT21600.017.8086131
ACGTCTG41500.017.69611715
AATCTCG41450.017.6513439
CACGTCT43200.017.40937414
TCTCGTA43950.016.57995441
ACGTTCG7650.016.5788676
CGTATCG21050.016.3939233
ATCTCGT45600.016.17430740
CCGTATC24450.015.8031692
TATCGAA23800.015.2431935
CACACGT50450.015.08305212
TCGGAAG49500.015.0752473
Produced by FastQC (version 0.11.5)