FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20716585
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTG759950.36683169547490574TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG97200.050.7494145
TATGCCG100450.049.1722348
CTCGTAT100900.048.83088744
CGTATGC103450.047.71267346
ACGTCTG110650.045.4194415
GTATGCC108800.045.37132347
ACACGTC113200.044.6048413
CACGTCT116600.043.27878614
GCCGTCT114050.041.6497651
CACACGT123350.041.0301212
TCTCGTA121750.040.61357543
ATCTCGT123000.040.1758842
TCGGAAG128900.039.265873
GATCGGA130950.038.5754551
AATCTCG128400.038.37070541
GCACACG133500.038.1757111
CGTCTGA133350.037.9091916
GTCAACA132000.037.72516635
CGGAAGA138350.036.6051334
AGTCAAC136900.036.41490634
Produced by FastQC (version 0.11.5)