FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25529237
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32750.042.87725448
TCGTATG34850.040.54495645
CGTATGC38350.037.07554246
TCCGTAT42650.032.71481737
CTCGTAT43350.032.39069444
TATCTCG47800.029.2523941
CGTATCT50650.028.0723339
GTTCCGT52900.026.7097835
GATCGGA55050.025.706041
GTATGCC55200.025.49239547
CGTCTGA55750.025.44987316
TTCCGTA57550.024.34662636
ACGTCTG59700.023.86482215
ACACGTC62000.023.07466913
CCGTATC65900.021.53131738
TCTCGTA67300.021.17107843
CACGTCT69800.020.70742214
AGTTCCG70150.020.4779634
TCGGAAG73600.019.4798073
ATCTCGT75650.018.7952942