FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15830857
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTG261870.1654174502365854TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37250.043.8724445
TATGCCG37750.043.21593548
CGTATGC41500.039.5216446
GATCGGA43600.038.016291
CTCGTAT44000.037.0079244
ACCCGTC45150.035.93281632
TCCCGAT47200.034.5614137
CGTCCCG47150.034.47216835
GTATGCC48050.034.3205447
CGTCTGA48700.033.97986216
ACACGTC49450.033.4643913
GTCCCGA50200.032.73031236
ACGTCTG51200.032.26307715
CACCCGT52450.030.87544331
CCCGATC56050.028.84137538
CACGTCT57550.028.80571714
CCGATCT56150.028.7900139
ATCGGAA58200.028.5869052
ATGCCGT58050.028.35748349
CACACGT61550.027.12530912
Produced by FastQC (version 0.11.5)