FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17340998
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG11150.016.403144
CGTTTCG13800.014.96250332
TATGCCG12750.014.11387647
CCCGTAT13900.012.3279691
CGTATCG14400.011.8821053
ACGTTCG11000.011.2639516
GTATCGA8650.011.2546494
CTCGTAT17300.010.91300143
CGGTATA13400.010.56627211
CGTATGC17050.010.55393945
TCGACCG12900.010.51849131
GCGATCG12100.010.23939441
TCGCGTG4359.803625E-810.1720242
ATTCGAC11700.010.08476435
GTTCGAC14650.09.86623129
TTACGAC7200.09.8336095
CCCGTCT47200.09.7647461
GTCGTAT5901.02591E-99.5143671
CGAGCGA19900.09.48789453
CGACCGT14350.09.25008932
Produced by FastQC (version 0.11.5)