FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21688493
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTG264370.12189413068026442TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44200.044.5844948
TCGTATG45450.043.6150145
CGTATGC49550.040.00628346
CTCGTAT51150.038.4663344
GTATGCC59100.033.49381647
ACGTCTG65650.031.0925215
TCTCGTA64850.030.75015443
ACACGTC67200.030.24367113
CACGTCT71400.028.87776214
TATCTCG68400.028.72292941
GATCGGA71450.028.5319771
CGTCTGA73150.028.10625516
ATCTCGT74500.026.72748442
CACACGT82850.024.85120612
TCGGAAG82550.024.7657093
GCCGTCT76200.024.00243651
ATCGGAA94700.021.6182792
CGGAAGA95150.021.486674
ATGCCGT93150.021.47219749
TTATCTC94800.021.19089740
Produced by FastQC (version 0.11.5)