FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12840643
Sequences flagged as poor quality0
Sequence length65
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAA3430462.6715640330472548TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAA553280.4308818491410438TruSeq Adapter, Index 2 (100% over 62bp)
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAAT241360.1879656649593015No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTA227990.1775534137971128No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAAATGTTGAGCCG222990.17365952779779018No Hit
GTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTA216790.16883110915863014No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTG191430.1490813193700658No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACCCA181520.14136363731940838No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGG174530.13591998469235536No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGT173490.13511005640449625No Hit
CTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAG168720.1313952891611425No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGG166290.1295028605654717No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTC164300.12795309393774126No Hit
GGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCT164000.12771946077778193No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTA163590.12740016212583746No Hit
GTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGCTCATGAGTG158930.12377106037446879No Hit
CCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTA158870.12372433374247692No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTG154940.12066373934700933No Hit
GTAAAGGATGCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGA153170.11928530370324912No Hit
GTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG149740.1166140979077138No Hit
GTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGC149730.11660631013571515No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCCTCCTA148620.11574186744386554No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGTAGAGTTTG148370.11554717314389941No Hit
GGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG145430.1132575681762977No Hit
GGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTG144920.11286039180436681No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAA140720.10958952756493581No Hit
CTTTAGTGTTGTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGGTGGTG132470.10316461566605348No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGGTTAGGCGTACGGCCA131160.10214441753423095No Hit
CTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTC129080.10052456095851275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA465500.050.037711
CTTGAAA79800.049.68385759
GCCGTGA10000.049.26580449
ACACGTC471250.049.0059113
CTCGTAT465400.048.9143142
GTATCTC465100.048.90780638
ACGTCTG472150.048.87501515
CACGTCT472400.048.87412614
TATCTCG465550.048.86687539
TATGCCG467250.048.8469146
TCGTATG467850.048.8410243
TGTATCT466000.048.8191137
TCACCGA467750.048.81133730
CAGTCAC468000.048.80072427
GAACTCC470000.048.792221
CGTATGC468100.048.78323444
CCGTGAT10100.048.7780250
GTCACCG468200.048.77091629
ACCGATG468100.048.76223832
AACTCCA469100.048.76174522
Produced by FastQC (version 0.11.5)