FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21340946
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAA238570.11178979600998007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17000.033.3230346
TCGTATG17800.031.98609543
CGTATGC22900.024.73553344
CTCGTAT24850.023.267642
GATCGGA29300.019.9610611
GTATGCC31250.018.40941845
ACACGTC34250.017.6547813
ACGTCTG36300.016.0890215
CGTCTGA36850.016.00897616
ACGTTCG20050.014.5667996
ACTTAGG44550.014.16889832
ATGCCGT44450.013.93925947
CACTTAG44650.013.67473431
CACGTCT45100.013.407454514
GCGATCG21400.013.23302341
TCGGAAG45800.013.2043313
CGTTCGA23350.013.0129667
CGTACGA8500.012.84156554
GACCGCA114150.012.58445119
GTCCGAT13900.012.5378551
Produced by FastQC (version 0.11.5)