FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22643095
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24800.017.38891
ACGTTCG17450.016.5684686
GTATCGA19050.015.9512574
CGTATCG27100.014.3696373
CGTTCGA20150.014.3479017
GCGATCG20650.014.1419241
GTCCGAT14050.013.6649481
TATGCCG11900.013.636620546
TATCGAA24300.013.3548525
CGCTAGT4455.456968E-1212.6114361
TCGTATG13000.012.48076943
TCGACCG25100.012.221621531
CCGTATC35050.012.2045592
GTATACG18350.011.89501212
CGACCGT26200.011.70850132
ACGCTTA23900.011.6033344
TATACGG19100.011.58236213
TTACCGG12600.011.471318
TTACGAC11900.011.4041665
GTTCGAC27050.011.34063229
Produced by FastQC (version 0.11.5)