FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18797057
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAA629080.3346694112807127TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG94850.048.11527643
TATGCCG96200.047.446546
CTCGTAT97700.046.68101542
CGTATGC99400.045.8263444
GTATGCC105150.043.5167845
ACGATCA108200.042.4451332
AGTCACG110050.041.91921628
GTCACGA111100.041.36374329
ACGTCTG115300.040.47118815
CACGATC114700.040.11692431
TCACGAT115950.039.786230
ACACGTC120800.038.67727713
CACGTCT122800.038.21549214
GATCGGA124700.037.7781751
ATCTCGT126500.036.19133840
GCCGTCT117650.035.9115649
TCTCGTA127800.035.66152241
CGTCTGA131550.035.4046316
CACACGT132600.035.2354112
CGATCAG133150.034.5832933
Produced by FastQC (version 0.11.5)