FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14690838
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13150.027.82198746
TCGTATG13300.027.28246543
CGTATGC16600.021.85995744
CTCGTAT18600.019.19108842
TATCTCG20050.018.391839
GATCGGA22600.017.2522561
CGTCTGA23750.016.02116616
ACGTCTG24750.015.37384615
TAGCTTA24600.015.34982834
GTATGCC23850.015.21489845
ACTAGCT25650.014.94925632
ACGTTCG14250.014.2848186
CACTAGC28550.013.63739531
GCTTATC27350.013.59070136
ACACGTC29050.013.30118213
ATGCCGT32350.012.22144947
CGTTCGA16950.012.1829527
CGCATCG11200.012.11625638
GCGATCG17700.011.8329141
CACGTCT32850.011.76253714
Produced by FastQC (version 0.11.5)