FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16444687
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10900.028.15046546
TCGTATG12450.024.87951343
CGTATGC15150.020.83599544
CTCGTAT17500.017.86835342
CGGCTAC18000.017.3712733
ACGGCTA19200.016.74540732
GTATGCC23100.014.17601245
CCCGTAT11750.013.0711361
CGCATCG12250.013.00388238
TCGACCG18600.012.84524831
ACGTTCG15200.012.2279026
AGTCACG26250.012.02351828
GATCGGA27150.011.9665991
GTTCGAC20950.011.82679429
CGACCGT20400.011.71184432
GTCCGAT11100.011.7078621
ACGTCTG28550.011.67472715
ACACGTC28250.011.48536113
CGTTCGA16500.011.442917
ACGTACG6500.011.34660153
Produced by FastQC (version 0.11.5)