FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21106007
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18450.029.1016745
TATGCCG22450.023.78651648
CGTATGC26850.020.21842446
CTCGTAT29350.018.3944144
ACGTTCG20650.017.8577046
GTATCGA19150.016.4836244
GTATGCC33500.016.02877647
GATCGGA37150.015.4247121
CCCGTAT26500.015.3818071
CGTTCGA24350.015.2646447
ACGTCTG37650.015.12061115
ACACGTC40550.013.89365313
CGTATCG27800.013.7950723
TATCGAA23250.013.7037215
GCGATCG26900.013.59800741
GTCCGAT13350.013.496551
CACGTCT46350.012.91874214
TCGACCG27400.012.91824631
CGTCTGA45000.012.84755716
TTCGCGC9250.012.7565547
Produced by FastQC (version 0.11.5)