FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12653570
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTG288050.2276432658925505TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG41900.047.1752645
TATGCCG42850.046.13173748
TCCGTAT47350.043.67359537
CGTATGC45450.043.4286846
CTCGTAT45700.042.99436644
TATCTCG46950.041.78238341
CGTATCT48550.040.58897839
GTTCCGT51250.040.58038335
GTATGCC50250.039.39769747
ACGTCTG53900.038.69072715
CGTCTGA55850.037.33984416
ACACGTC57600.036.205113
CACGTCT60800.034.63916814
GATCGGA60750.034.279861
GCCGTCT56550.032.76289451
CCGTATC63400.032.33824538
CACACGT65750.031.89676912
ATGCCGT63850.031.00565149
TTCCGTA67000.030.99693536
GTATCTC64900.030.31654440
Produced by FastQC (version 0.11.5)