FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17455718
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31250.025.67876448
TCGTATG33300.024.36288645
GATCGGA35600.023.2296431
CGTATGC35200.023.04764746
CCCGTAT16000.021.4127541
GTATCGA12800.020.279784
CTCGTAT41750.019.36018444
GTATGCC43750.018.67914247
GTCCGCA44450.018.38387734
ACGTCTG45200.018.20728115
ACGTCCG45600.018.17824432
CGTATCG17150.018.0601223
AGTCACG47400.017.5492128
ACACGTC48050.017.3733513
TCCGCAC47250.017.35704635
CGCACAT47300.017.27632937
CGTCCGC47650.017.14908633
CGTCTGA49750.016.60133616
CCGTATC22650.015.8887912
CACGTCT53550.015.5885714
Produced by FastQC (version 0.11.5)