FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14519648
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC10800.021.03051431
TATGCCG11750.019.58411647
CGTACGT13250.018.03407933
TACGTAA12750.017.81598535
TCGTATG13000.017.69980844
GTACGTA13950.016.91767134
CCGTACG14450.016.74013132
CCCGTAT12950.016.1938151
ACGTAAT14400.015.56970536
CGTATGC14850.015.29671245
CGTAATC15200.015.13841237
CACCGTA16900.014.13778130
CTCGTAT17500.013.65413943
ACGTTCG15150.013.4346656
CGACCGT19850.013.07780932
TCGACCG19200.013.05872131
GTTCGAC20000.012.83125629
GTATCGA8100.012.746034
GCGATCG15700.012.40131241
CGTTCGA16800.012.115197
Produced by FastQC (version 0.11.5)