FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19500381
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTG641950.32919869616906455TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGA271460.13920753650915846TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG119100.039.18663845
CTCGTAT119900.038.7751644
TATCTCG122850.038.06085241
TATGCCG125100.037.40247348
CGTATGC126650.036.89690446
GTATGCC130200.035.89208247
GATCGGA133100.035.7907641
ATATCTC131350.035.71104440
TGATATA133250.035.40128336
ACGTCTG135400.034.8562915
CACGTCT136700.034.80532514
ACACGTC139150.034.0028613
TCTCGTA137350.033.9778243
ATCTCGT138200.033.70514742
CTGATAT142500.033.10330235
CACACGT146600.032.51634612
GCCGTCT131900.032.0531251
ATATATC147300.031.90441538
ACTGATA151400.031.39100334
TCGGAAG153400.031.2093093
Produced by FastQC (version 0.11.5)