FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20480048
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAA496130.2422504087881044TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG79200.050.50702743
CTCGTAT83950.047.68476542
TATGCCG84950.047.0912446
CGTATGC88100.045.471744
ACGATCT95950.042.21401237
GTATGCC95450.041.97307645
ACGTCTG99400.041.33817315
ACACGTC104150.039.53858613
CACGATC103050.039.33415636
TCACGAT104050.038.9277735
CACGTCT110650.037.2685314
CGTCTGA114350.036.0619416
GCCGTCT92450.035.9648149
CACACGT117750.035.04707712
GATCGGA122450.034.181051
TCTCGTA120000.033.67912741
TCGGAAG130300.032.10133
ATGCCGT131400.031.65776647
GTCACAT130650.031.22408929
CGGAAGA141350.029.7168544
Produced by FastQC (version 0.11.5)