FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37260342
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68000.029.7177146
TCGTATG70400.028.66170543
CGTATGC74650.026.9508944
CTCGTAT80000.025.6651442
TATCTCG86050.023.82671439
GATCGGA87650.023.4870411
GTATGCC91950.022.20206345
TCACCGA97200.021.12152930
ACGTCTG101700.020.59309215
CGTCTGA102550.020.1058916
ACCGATG102900.019.98143432
ACACGTC107450.019.40919913
CACGTCT116150.018.18352114
CACCGAT117550.017.46501731
ATGCCGT126000.017.11562547
GATGTAT125700.016.52212135
ATCGGAA125000.016.4722042
CCGATGT127050.016.3928733
GCCGTCT124300.016.25797849
CGATGTA129150.016.1720934
Produced by FastQC (version 0.11.5)