FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568275
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14850.021.05657643
TATGCCG15500.020.7450646
CGTATGC20500.015.54111244
CCCGTAT19500.014.9968851
CGTATCG23000.013.7236943
GCGATCG16250.013.61465541
CTCGTAT23100.013.5361642
ACGTTCG16800.013.3448186
GTCCGAT16100.012.292761
CCGTATC27600.012.0777322
GTATCGA18150.012.0272234
TCGACCG14100.011.08795931
CGAATTG7150.010.7271013
ACGTCTG31100.010.43347715
CGTTCGA21300.010.3866747
TATCGAA25750.010.1958415
CGCGTTA14700.010.03394134
CGCATCG10850.09.78794838
CGATCGG23650.09.728929542
CGACCGT17850.09.58483532
Produced by FastQC (version 0.11.5)