FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15194316
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAA403680.26567829706845636TruSeq Adapter, Index 7 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAA259250.17062301455360018TruSeq Adapter, Index 7 (100% over 62bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80150.035.8558921
TCGTATG87300.031.76288843
TATGCCG91150.030.35765646
CGTATGC92550.029.961244
CTCGTAT92850.029.80007242
GTATGCC98450.028.22595845
ACGTCTG101400.027.78188915
ACACGTC103650.027.34956613
CACGTCT107700.026.26632114
CGTCTGA109500.025.91528116
CACACGT114050.025.08838812
ATCTCGT112900.024.7964840
GCCGTCT106850.024.79270449
TCGGAAG116200.024.6508753
ACCAGAT119500.023.52447332
ATGCCGT123650.023.45208547
CGGAAGA124550.023.0214774
TCTCGTA123500.022.49981141
GCACACG128750.022.45304111
CTTGAAA89400.022.1751659
Produced by FastQC (version 0.11.5)