FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10967722
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAA157900.14396790874166943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG11500.041.29966743
TATGCCG12800.037.567346
CGTATGC14100.034.3122544
CTCGTAT14800.031.89017942
TATCTCG17400.027.6343339
ACGTCTG19000.025.77206615
ACTAGCT18200.025.77035932
GTATGCC20350.024.06412745
GATCGGA21050.022.7321281
CGTCTGA21550.022.7224716
TAGCTTA21400.022.33118634
GCTTATC21550.022.31263736
ACACGTC22250.021.8750313
CACGTCT24900.020.02083214
TCACTAG23750.019.9968230
CACTAGC24000.019.6654331
TCGGAAG27300.017.9371743
CACACGT27550.017.66676512
CGGAAGA30000.016.6179714
GCCGTCT28450.015.55397349
Produced by FastQC (version 0.11.5)