FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240221
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT14350.017.4951231
CGTATCG16050.015.4391983
TCGTATG8100.015.29575943
GTATCGA13800.015.1773214
ACGTTCG12150.014.5675436
TATGCCG8550.014.49117746
TATCGAA15400.013.7920135
GCGATCG12850.013.54384241
CCGTATC20950.012.8138422
TTACCGG8200.012.5906678
GACCGCA62050.012.36047819
CGTTCGA13950.012.2644587
CGCTATG9550.012.04656929
TCGACCG12750.012.0305731
GTCCGAT9100.012.0090951
ACCGCAA65450.011.98926320
ATCGAAG30450.011.9159626
CGACCGT12950.011.84476932
CCGCAAT66800.011.83528721
CCCGTCT30400.011.4645871
Produced by FastQC (version 0.11.5)