FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21893266
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAA310370.14176505232248127TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG54900.035.40997743
TATGCCG59150.032.9166546
GATCGGA61450.032.106881
CGTATGC63250.030.7822244
CTCGTAT64900.029.81671342
GTATGCC70650.027.76706345
ACACGTC73950.026.76566513
ACGTCTG75600.025.90836515
CGTCTGA80350.024.41341416
CACGTCT82300.024.0142314
GCCGTCT83550.023.30364849
TCGGAAG87450.022.7028663
AATCTCG86650.022.60580439
CACACGT88550.022.45251712
CGGAAGA96700.020.8361114
TAATCTC96550.020.7156238
ATGCCGT101500.020.49035647
ATCTCGT98000.019.98768440
ATCGGAA99700.019.6766872
TCTCGTA106250.018.29587441
Produced by FastQC (version 0.11.5)