FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21807061
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG21300.033.6544645
TATGCCG22300.032.41015648
CGTATGC28150.025.98921846
CTCGTAT28900.025.11037444
ACGTCTG40250.018.39513215
GTATGCC40450.018.37815747
GATCGGA42550.017.5636941
ACACGTC43050.017.13017813
CACGTCT46900.016.03844814
CCCGTAT21600.015.453241
CGTCTGA53100.013.94360916
CACACGT55050.013.61041512
GTATCGA18600.013.4811484
TCGGAAG57300.013.3856553
AATCTCG55850.013.04586341
ATCTCGT58900.012.771028542
CGTATCG23950.012.6868153
TCTCGTA61450.012.48140243
ACGTTCG13950.012.0536776
ACGGTTA26000.011.91272212
Produced by FastQC (version 0.11.5)