FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19179319
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27300.034.57910548
TCGTATG28150.033.4294845
CGTATGC30650.030.7034146
GATCGGA35100.027.429961
CTCGTAT35050.027.01668744
CGTCCCG36600.025.4696735
GTATGCC38100.025.08686647
TCCCGAT38500.024.74908437
CGTCTGA39400.024.33209816
ACGTCTG40650.023.6565715
GTCCCGA42500.022.48916836
ACCCGTC44000.021.65420732
ACACGTC45550.021.24125513
CACGTCT47950.020.23960114
ATGCCGT48450.019.97130649
CCGATCT48900.019.36487239
CACCCGT51400.018.53667631
TCGGAAG55800.017.921853
CACACGT55400.017.51784912
ATCTCGT55050.017.25431642
Produced by FastQC (version 0.11.5)